Summary of section one
MIC by broth dilution method
Equipments
1-two pipettes 5ml & 1ml
2- ten test tubes six are empty & four are filled (one filled with M.O& another with 4ml A.B(number it 1 using glass pencil) & two filled with n.broth
Summary of steps:- All the exp THREE steps ONLY
1-number the 6 empty test tubes 2,3,4,5,+ve and –ve using the glass pencil
then by using the 5ml pipette transfer 2ml broth to all these 6 empty tubes
2-Two fold serial dilution of the A.B using 5ml pipette
Transfer 2ml of A.B. from test tube A (numbered as 1) to test tube 2 & shake
well then transfer 2ml. from test tube 2 to test tube 3 & shake well then
transfer 2ml from test tube 3 to test tube 4
Repeat till reaching tube no. 5 & reject 2ml from test tube 5.... don't forget
3-By using 1ml pipette transfer 0.2 ml of M.O to test tubes 1,2,3,4,5 & +ve conrtol
i.e to all tubes except -ve control
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Quiz on 1st section
1-MIC is the abbreviation of………& defined as……….0
2-A.B diluted by…….fold serial dilution in MIC determination by broth dilution methodology
3-MIC measured in the units of……….0
4-MIC = ………………in MIC determination by broth dilution method
5-The rule of +ve control in MIC determination by broth dilution is…….and it contain ……and……. And it shows…….after incubation while the –ve control rule is………and it contains…….only And it still………..after incubation
6-test tubes are incubated at…….۰c for……..hours in MIC determination by broth dilution method