Innumerate the bateriophage classification
write host cells in each type
-virulent
host cell lytic cell
-temperate
lysogenic cell
Innumerate the bateriophage classification
write host cells in each type
-virulent
host cell lytic cell
-temperate
lysogenic cell
]*Innumerate growth of animal viruses in lab &explain one of them
1-living animal
e.g rabbit,guinea pig,mice
in this method we studt immuno response as we inter virus in animal cells and observe the signs of disease
or killing the animal and examine its tissue
2-embryonated egg:the most common method
3-cell culture: recently used method
*innumerate morphology of viruses &example
1-helical virus
e.g tobacco mosaic
2-polyhedral virus
e.g polio,adeno virus
3-enveloped virus
enveloped helical:influnza
enveloped poly hedral:HSV
4-complex virus
e.g poxvirus_bacterio phage
*bacterio phage has 2 types innumerate them an explain one of them
1-virulent virus:
where bacterio phage attached to the host bacterial cell and injects its DNA leaving protien coat out side .the intered DNA control the genetic machine of the cell stimulating it to transcript the DNA and then capsid formed around DNA then the cell damage occure making rupture of cell and virus realesed
2-temperate virus:
the same but no rupture occured as DNA be integrated w the bacterial chromosome
Innumerate the req for viral growth and explaine anti bacterial agent
-temperature
-ph
-radiation
-detergents
-50%glycerol
-formaldhyde
-photodynamic inactivation
ether suscptiblity&lipid solubility
-anti bacterial agents
anti bacterial&anti biotics &sulphonamides have no effect on viruses
some anti viral drugs are developed
oxidizng agents most effect disinfectants e.x
H2O2&HCLO4
write advantage and disadvantage of cell culture method
adv.
-more convenient
-homogenous cell colection
-recent method
disadv
-must be kept away from contamination
-need anti biotics to prevent contamination
-needs experienced workers
-identification of viral isolates not easy
define
1-tumor
cell muliplication in an un controled way
2-malignant cell
multiply in an un controlled way
3-leukomia
in crease in white blood cells
4-cpE
inhebition of protein or DNAsynthesis
*glycerol &formaldhyde have different effect on viruses explain
1-50%glycerol:virus are alive in 50%glycerol or many years e.g vaccine virus preserved in 50%glycerol for many years
2-formaldehyde
lead to loss of infectivity of virus but has minimal adverse effect an antigenicity and used in preparatn of non infective viral particles
*how can cancer be an infective diseas
يعنى ازاى ممكن ان يصبح السرطان مرض معدى
by altering genetic material of cell to cancerous cell
by chemicals,radiation as
reseptor gene form ecogen(1,2,3 chromosome)
reprosser protien block ecogen
but due to presence of viral gene and its protien which inerfer with reposser protien and lead to block reprosser gene and so ecogen become functional
interferon is not the anti viral agent ,but it induces it explain how
as it synthes other protien which inhibitRNA translation and protein synthesis (INHIBITviral replicatn)
وهنقول الميكانزم هحل بقيت الاسئله وأرجعها عشان مش هخلص كده
]define
1-tumor
cell muliplication in an un controled way
2-malignant cell
multiply in an un controlled way
هيه الخليه اللى بيحصل فيها الكلام الحلو اللى قولتيه ده![]()
3-leukomia
in crease in white blood cells
نقول برضه إنها بتعنى سرطان الدم
4-cpE
جميل جدا بس لازم نقول إنها cyto pathic effect ونكمل يا قلبى
inhebition of protein or DNAsynthesis ]
INCLUSION bodies impotance??
uesed in identification of cetain virus causing in fection
complete
1-viruses can survive in certain condition as..........
90-درجة مئوية
وطبعا الميكانيزم بتاع الانرفيرون مهم بس انا مش هكتبه
لان كلنا عارفينه
انا كدة خلصت الاسئلة بجد كانت جميييييييييييييييييلة ومتشعبة وحسيت انى لميت الموضوع الحمد لله ربنا يكرمك ويجزيكى كل خييييييييييييير
complete
*viruses are alive when they enter.in host cell
*host range are plant,bacteria,animal
*units that form capsid are called capsomer0
*viruses may be helical such astobacoo mosaic0
* host cell for virulent phage is lytic cell
*viruses can live in 5-9PHrange ,but all viruses destroyed in alkalinemedium
*influenza stabilized byMgso4salt [/quote]