ازيكوا يا دكاترة اولا
اخبار بحث الابليد ايييييييييييييييييه
انا البحث بتاعى عن chemical tests of bogbean
وللاسف مش لاقيه حاجه خااااااااااااااااااااااالص اعمل اييييييييييييه :fop (28):
عرض للطباعة
ازيكوا يا دكاترة اولا
اخبار بحث الابليد ايييييييييييييييييه
انا البحث بتاعى عن chemical tests of bogbean
وللاسف مش لاقيه حاجه خااااااااااااااااااااااالص اعمل اييييييييييييه :fop (28):
IDENTIFICATION
Thin layer chromatography).
Test solution. Mother tincture.
Reference solution. Dissolve 5 mg of loganine R and 5 mg of catalpol R in 15 mL of methanol R.
Plate: TLC silica gel plate R.
Mobile phase: water R, methanol R, ethyl acetate R (8:15:77 V/V/V).
Application: 30 μL, as bands.
Development: over a path of 15 cm.
Drying: in air.
Detection: spray with vanillin reagent R. Heat at 100-105 °C for 10 min. Examine in daylight.
Results: see below the sequence of zones present in the chromatograms obtained with the
reference solution and the test solution. Furthermore other faint zones may be present in the
chromatogram obtained with the test solution.
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ASSAYاقتباس:
المشاركة الأصلية كتبت بواسطة sara yousif
Ultraviolet and visible absorption spectrophotometry (2.2.25).
Test solution. In a 100.0 mL volumetric flask, place 5.000 g of mother tincture accurately weighed
and dilute with methanol R. In a round-bottomed flask, place 1.0 mL of this solution and evaporate
to dryness, under reduced pressure. Dissolve in 5.0 mL of a solution obtained with a mixture of 0.5
mL of a solution of ferric chloride (10 per cent m/V) R and 100 mL of sulfuric acid (50 per cent V/V)
R. Heat on a water-bath at 80 °C for 3 min so the coloration can develop.
Reference solution. In a 10.0 mL volumetric flask, place 2.0 mg of catalpol R accurately weighed
and dilute with methanol R. In a round-bottomed flask, place 1.0 mL of this solution and evaporate
to dryness under reduced pressure. Dissolve in 5.0 mL of a solution obtained with a mixture of 0.5
mL of a solution of ferric chloride (10 per cent m/V) R and 100 mL of sulfuric acid (50 per cent V/V)
R. Heat on a water-bath at 80 °C for 3 min so the coloration can develop.
Compensation liquid. In a round-bottomed flask, place 1.0 mL of methanol R and evaporate to
dryness. Dissolve in 5.0 mL of a solution obtained with a mixture of 0.5 mL of a solution of ferric
chloride (10 per cent m/V) R and 100 mL of sulfuric acid (50 per cent V/V) R. Heat on a water-bath
at 80 °C for 3 min so the coloration can develop.
Measure the absorbance of the test solution and the reference solution at 411 nm in comparison
with the compensation liquid.
Calculate the percentage content m/m of total iridoids, expressed as catalpol from the expression:
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http://ansm.sante.fr/var/ansm_site/s...a3bd1d1073.pdf
شكرا اوووووووووووووووووووووووى
مش عارفه اشكرك ازاى
انا البجث بتاعي عن
proximate analysis (ash value (total ash, acid insoluble ash,water soluble ash),extractive values and
moisture content (loss drying
لل parsley piert ومش لاقية حاجة خالص مش عارفة اعمل ايه حد يساعدني بليز :fop10
Ash value:
Ash remaining following ignition of medicinal plant
Total ash: Not more than 13.0 per cent
.
Acid insoluble ash: not more than 5.3%
Water soluble ash: not more than 6.3%
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Moisture content(loss on drying):about 65% content of moisture
---------- Post added at 06:42 PM ---------- Previous post was at 06:39 PM ----------
Extractive value: It contains not less than 2.1 per cent of parsley piert principle